PUBLICATIONS RELATED TO THE IIMENA PROJECT:

1. Whitford, C.M., Gockel, P., Faurdal, D., Gren, T., Sigrist, R., and Weber, T. (2025). CASCADE-Cas3 enables highly efficient genome engineering in Streptomyces species. Nucleic Acids Res. 53. DOI: 10.1093/nar/gkaf214.

2. Pakkir Shah, A.K., Walter, A., Ottosson, F., Russo, F., Navarro-Diaz, M., Boldt, J., Kalinski, J.J., Kontou, E.E., Elofson, J., Polyzois, A., et al. (2025). Statistical analysis of feature-based molecular networking results from non-targeted metabolomics data. Nat. Protoc. 20, 92-162. DOI: 10.1038/s41596-024-01046-3.

3. Mohite, O.S., Jorgensen, T.S., Booth, T.J., Charusanti, P., Phaneuf, P.V., Weber, T., and Palsson, B.O. (2025). Pangenome mining of the Streptomyces genus redefines species’ biosynthetic potential. Genome Biol. 26, 9. DOI: 10.1186/s13059-024-03471-9.

4. Pakkir Shah, A.K., Walter, A., Ottosson, F., Russo, F., Navarro-Diaz, M., Boldt, J., Kalinski, J.J., Kontou, E.E., Elofson, J., Polyzois, A., et al. (2024). Statistical analysis of feature-based molecular networking results from non-targeted metabolomics data. Nat. Protoc. DOI: 10.1038/s41596-024-01046-3.

5. Ortiz-Lopez, F.J., Oves-Costales, D., Guerrero Garzon, J.F., Gren, T., Baggesgaard Sterndorff, E., Jiang, X., Sparholt Jo Rgensen, T., Blin, K., Fernandez-Pastor, I., Tormo, J.R., et al. (2024). Genome-Led Discovery of the Antibacterial Cyclic Lipopeptide Kutzneridine A and Its Silent Biosynthetic Gene Cluster from Kutzneria Species. J. Nat. Prod. 87, 2515-2522. DOI: 10.1021/acs.jnatprod.4c00633.

6. Nuhamunada, M., Mohite, O.S., Phaneuf, P.V., Palsson, B.O., and Weber, T. (2024). BGCFlow: systematic pangenome workflow for the analysis of biosynthetic gene clusters across large genomic datasets. Nucleic Acids Res. 52, 5478-5495. DOI: 10.1093/nar/gkae314.

7. Mohite, O.S., Jørgensen, T.S., Booth, T.J., Charusanti, P., Phaneuf, P.V., Weber, T., and Palsson, B.O. (2024). Pangenome mining of the Streptomyces genus redefines their biosynthetic potential. BioRxiv, DOI: 10.1101/2024.1102.1120.581055. DOI: 10.1101/2024.02.20.581055.

8. Maleckis, M., Wibowo, M., Williams, S.E., Gotfredsen, C.H., Sigrist, R., Souza, L.D.O., Cowled, M.S., Charusanti, P., Gren, T., Saha, S., et al. (2024). Maramycin, a Cytotoxic Isoquinolinequinone Terpenoid Produced through Heterologous Expression of a Bifunctional Indole Prenyltransferase/Tryptophan Indole-Lyase in S. albidoflavus. ACS Chem. Biol. 19, 1303-1310. DOI: 10.1021/acschembio.4c00121.

9. Maleckis, M., Wibowo, M., Williams, S.E., Gotfredsen, C.H., Sigrist, R., Souza, L.D.O., Cowled, M.S., Charusanti, P., Gren, T., Saha, S., et al. (2024). Maramycin, a cytotoxic isoquinolinequinone terpenoid produced through heterologous expression of a bifunctional indole prenyltransferase /tryptophan indole-lyase in S. albidoflavus. BioRxiv. DOI: 10.1101/2024.02.14.580309.

10. Maleckis, M., Wibowo, M., Gren, T., Jarmusch, S.A., Sterndorff, E.B., Booth, T., Henriksen, N., Whitford, C.M., Jiang, X., Jorgensen, T.S., et al. (2024). Biosynthesis of the Azoxy Compound Azodyrecin from Streptomyces mirabilis P8-A2. ACS Chem. Biol. 19, 641-653. DOI: 10.1021/acschembio.3c00632.

11. Jørgensen, T.S., Mohite, O.S., Sterndorff, E.B., Alvarez-Arevalo, M., Blin, K., Booth, T.J., Charusanti, P., Faurdal, D., Hansen, T.O., Nuhamunada, M., et al. (2024). A treasure trove of 1034 actinomycete genomes. Nucleic Acids Res. 52, 7487-7503. DOI: 10.1093/nar/gkae523.

12. Jørgensen, T.S., Mohite, O., Sterndorff, E.B., Alvarez-Arevalo, M., Blin, K., Booth, T.J., Charusanti, P., Faurdal, D., Hansen, T.O., Nuhamunada, M.J., et al. (2024). A treasure trove of 1,034 actinomycete genomes. BioRxiv, DOI: 10.1101/2024.1101.1116.574955. DOI: 10.1101/2024.01.16.574955.

13. Fernandez-Pastor, I., Ortiz-Lopez, F.J., Oves-Costales, D., Martin, J., Sanchez, P., Melguizo, A., Reyes, F., Weber, T., and Genilloud, O. (2024). Dilarmycins A-C, Calcium-Dependent Lipopeptide Antibiotics with a Non-canonical Ca(2+)-Binding Motif. Org. Lett. 26, 1343-1347. DOI: 10.1021/acs.orglett.3c04195.

14. Blin, K., Shaw, S., Medema, M.H., and Weber, T. (2024). The antiSMASH database version 4: additional genomes and BGCs, new sequence-based searches and more. Nucleic Acids Res. 52, D586-D589. DOI: 10.1093/nar/gkad984.

15. Yin, K., Cruz-Morales, P., Whitford, C.M., and Keasling, J.D. (2023). Heterologous production of polycyclopropanated fatty acids and their methyl esters in Streptomyces. STAR Protoc 4, 102190. DOI: 10.1016/j.xpro.2023.102190.

16. Whitford, C.M., Gren, T., Palazzotto, E., Lee, S.Y., Tong, Y., and Weber, T. (2023). Systems Analysis of Highly Multiplexed CRISPR-Base Editing in Streptomycetes. ACS Synth Biol 12, 2353-2366. DOI: 10.1021/acssynbio.3c00188.

17. Whitford, C.M., Gockel, P., Faurdal, D., Gren, T., Sigrist, R., and Weber, T. (2023). CASCADE-Cas3 Enables Highly Efficient Genome Editing in Streptomyces species. BioRxiv, 2023.2005.2009.539971. DOI: https://doi.org/10.1101/2023.05.09.539971.

18. Terlouw, B.R., Blin, K., Navarro-Munoz, J.C., Avalon, N.E., Chevrette, M.G., Egbert, S., Lee, S., Meijer, D., Recchia, M.J.J., Reitz, Z.L., et al. (2023). MIBiG 3.0: a community-driven effort to annotate experimentally validated biosynthetic gene clusters. Nucleic Acids Res. 51, D603-D610. DOI: 10.1093/nar/gkac1049.

19. Sharma, K., Ghiffary, M.R., Lee, G., and Kim, H.U. (2023). Efficient production of an antitumor precursor actinocin and other medicinal molecules from kynurenine pathway in Escherichia coli. Metab. Eng. DOI: 10.1016/j.ymben.2023.11.008.

20. Pakkir Shah, A.K., Walter, A., Ottosson, F., Russo, F., Navarro-Díaz, M., Boldt, J., Kalinski, J.-C., Kontou, E.E., Elofson, J., Polyzois, A., et al. (2023). The Hitchhiker’s Guide to Statistical Analysis of Feature-based Molecular Networks from Non-Targeted Metabolomics Data. ChemRxiv (PREPRINT, NOT PEER REVIEWED), DOI: 10.26434/chemrxiv-22023-wwbt26430. DOI: 10.26434/chemrxiv-2023-wwbt0.

21. Oves-Costales, D., Gren, T., Sterndorff, E.B., Martín, J., Ortiz-López, F.J., Jørgensen, T.S., Jiang, X., Román-Hurtado, F., Reyes, F., Genilloud, O., and Weber, T. (2023). Identification and heterologous expression of the globomycin biosynthetic gene cluster. Synth. Syst. Biotechnol. 8, 206-212. DOI: 10.1016/j.synbio.2023.02.001.

22. Ortiz-Lopez, F.J., Oves-Costales, D., Carretero-Molina, D., Martin, J., Diaz, C., de la Cruz, M., Roman-Hurtado, F., Alvarez-Arevalo, M., Jorgensen, T.S., Reyes, F., et al. (2023). Crossiellidines A-F, Unprecedented Pyrazine-Alkylguanidine Metabolites with Broad-Spectrum Antibacterial Activity from Crossiella sp. Org. Lett. 25, 3502-3507. DOI: 10.1021/acs.orglett.3c01088.

23. Nuhamunada, M., Mohite, O.S., Phaneuf, P.V., Palsson, B., and Weber, T. (2023). BGCFlow: Systematic pangenome workflow for the analysis of biosynthetic gene clusters across large genomic datasets. BioRxiv. DOI: 10.1101/2023.06.14.545018.

24. Maleckis, M., Wibowo, M., Gren, T., Jarmusch, S.A., Sterndorff, E.B., Booth, T.J., Henriksen, N.N.S.E., Whitford, C.M., Jiang, X., Jørgensen, T.S., et al. (2023). Biosynthesis of the azoxy compound azodyrecin from Streptomyces mirabilis P8-A2. BioRxiv, https://doi.org/10.1101/2023.1110.1113.562208. DOI: https://doi.org/10.1101/2023.10.13.562208.

25. Kontou, E.E., Walter, A., Alka, O., Pfeuffer, J., Sachsenberg, T., Mohite, O.S., Nuhamunada, M., Kohlbacher, O., and Weber, T. (2023). UmetaFlow: an untargeted metabolomics workflow for high-throughput data processing and analysis. J Cheminform 15, 52. DOI: 10.1186/s13321-023-00724-w.

26. Jørgensen, T.S., Pedersen, M.S., Blin, K., Kuntke, F., Salling, H.K., Marvig, R.L., Michaelsen, T.Y., Albertsen, M., and Larsen, H. (2023). SpikeSeq: A rapid, cost efficient and simple method to identify SARS-CoV-2 variants of concern by Sanger sequencing part of the spike protein gene. J. Virol. Methods 312, 114648. DOI: 10.1016/j.jviromet.2022.114648.

27. Faurdal, D., Jorgensen, T.S., Alvarez-Arevalo, M., Mourched, A.S., Sterndorff, E.B., Charusanti, P., and Weber, T. (2023). Complete Genome Sequences of the Two Strains Methylorubrum extorquens NBC_00036 and NBC_00404. Microbiol Resour Announc 12, e0011523. DOI: 10.1128/mra.00115-23.

28. Dolya, B., Hryhorieva, O., Sorochynska, K., Lopatniuk, M., Ostash, I., Tseduliak, V.-M., Sterndorff, E.B., Jørgensen, T.S., Gren, T., Dacyuk, Y., et al. (2023). Properties of Multidrug-Resistant Mutants Derived from Heterologous Expression Chassis Strain Streptomyces albidoflavus J1074. Microorganisms 11. DOI: 10.3390/microorganisms11051176.

29. Blin, K., Shaw, S., Augustijn, H.E., Reitz, Z.L., Biermann, F., Alanjary, M., Fetter, A., Terlouw, B.R., Metcalf, W.W., Helfrich, E.J.N., et al. (2023). antiSMASH 7.0: new and improved predictions for detection, regulation, chemical structures and visualisation. Nucleic Acids Res. 51, W46-W50. DOI: 10.1093/nar/gkad344.

30. Alvarez-Arevalo, M., Sterndorff, E.B., Faurdal, D., Mourched, A.S., Charusanti, P., Jørgensen, T.S., and Weber, T. (2023). Complete, circular genome sequence of a Bosea sp. isolate from soil. Microbiol Resour Announc, e0036023. DOI: 10.1128/MRA.00360-23.

31. Alvarez-Arevalo, M., Sterndorff, E.B., Faurdal, D., Jørgensen, T.S., Mourched, A.S., Vuksanovic, O., Saha, S., and Weber, T. (2023). Extraction and Oxford Nanopore sequencing of genomic DNA from filamentous Actinobacteria. STAR Protoc 4, 101955. DOI: 10.1016/j.xpro.2022.101955.

32. van ‘t Hof, M., Mohite, O.S., Monk, J.M., Weber, T., Palsson, B.O., and Sommer, M.O.A. (2022). High-quality genome-scale metabolic network reconstruction of probiotic bacterium Escherichia coli Nissle 1917. BMC Bioinformatics 23, 566. DOI: 10.1186/s12859-022-05108-9.

33. Sterndorff, E.B., Jørgensen, T.S., Garzon, J.F.G., Schmid, J., Grond, S., and Weber, T. (2022). Complete Genome Sequence of the Collinolactone Producer Streptomyces sp. Strain Go40/10. Microbiol Resour Announc 11, e0022022. DOI: 10.1128/mra.00220-22.

34. Sanchéz-Navarro, R., Nuhamunada, M., Mohite, O.S., Wasmund, K., Albertsen, M., Gram, L., Nielsen, P.H., Weber, T., and Singleton, C.M. (2022). Long-Read Metagenome-Assembled Genomes Improve Identification of Novel Complete Biosynthetic Gene Clusters in a Complex Microbial Activated Sludge Ecosystem. mSystems, e0063222. DOI: 10.1128/msystems.00632-22.

35. Nielsen, J.B., Gren, T., Mohite, O.S., Jørgensen, T.S., Klitgaard, A.K., Mourched, A.S., Blin, K., Oves-Costales, D., Genilloud, O., Larsen, T.O., et al. (2022). Identification of the Biosynthetic Gene Cluster for Pyracrimycin A, an Antibiotic Produced by Streptomyces sp. ACS Chem. Biol. 17, 2411-2417. DOI: 10.1021/acschembio.2c00480.

36. Mungan, M.D., Blin, K., and Ziemert, N. (2022). ARTS-DB: a database for antibiotic resistant targets. Nucleic Acids Res. 50, D736-D740. DOI: 10.1093/nar/gkab940.

37. Mohite, O.S., Lloyd, C.J., Monk, J.M., Weber, T., and Palsson, B.O. (2022). Pangenome analysis of Enterobacteria reveals richness of secondary metabolite gene clusters and their associated gene sets. Synth Syst Biotechnol 7, 900-910. DOI: 10.1016/j.synbio.2022.04.011.

38. Kontou, E.E., Walter, A., Alka, O., Pfeuffer, J., Sachsenberg, T., Mohite, O., Nuhamunada, M., Kohlbacher, O., and Weber, T. (2022). UmetaFlow: An untargeted metabolomics workflow for high-throughput data processing and analysis. ChemRxiv, DOI: 10.26434/chemrxiv-22022-z26430t26434g-v26432. DOI: 10.26434/chemrxiv-2022-z0t4g-v2.

39. Jorgensen, T.S., Pedersen, M.S., Blin, K., Kuntke, F., Salling, H.K., Marvig, R.L., Michaelsen, T.Y., Albertsen, M., and Larsen, H. (2022). SpikeSeq: A rapid, cost efficient and simple method to identify SARS-CoV-2 variants of concern by Sanger sequencing part of the spike protein gene. J. Virol. Methods 312, 114648. DOI: 10.1016/j.jviromet.2022.114648.

40. Jarmusch, A.K., Aron, A.T., Petras, D., Phelan, V.V., Bittremieux, W., Acharya, D.D., Ahmed, M.M.A., Bauermeister, A., Bertin, M.J., Boudreau, P.D., et al. (2022). A Universal Language for Finding Mass Spectrometry Data Patterns. BioRxiv. DOI: 10.1101/2022.08.06.503000.

41. Carretero-Molina, D., Ortiz-López, F.J., Gren, T., Oves-Costales, D., Martín, J., Román-Hurtado, F., Sparholt Jørgensen, T., de la Cruz, M., Díaz, C., Vicente, F., et al. (2022). Discovery of gargantulides B and C, new 52-membered macrolactones from Amycolatopsis sp. Complete absolute stereochemistry of the gargantulide family. Organic Chemistry Frontiers 9, 462-470. DOI: 10.1039/d1qo01480c.

42. Yang, D., Jang, W.D., and Lee, S.Y. (2021). Production of Carminic Acid by Metabolically Engineered Escherichia coli. J. Am. Chem. Soc. 143, 5364-5377. DOI: 10.1021/jacs.0c12406.

43. Whitford, C.M., Cruz-Morales, P., Keasling, J.D., and Weber, T. (2021). The Design-Build-Test-Learn cycle for metabolic engineering of Streptomycetes. Essays Biochem. 65, 261-275. DOI: 10.1042/EBC20200132.

44. Tong, Y., Jørgensen, T.S., Whitford, C.M., Weber, T., and Lee, S.Y. (2021). A versatile genetic engineering toolkit for E. coli based on CRISPR-prime editing. Nat. Commun. 12, 5206. DOI: 10.1038/s41467-021-25541-3.

45. Steinke, K., Mohite, O.S., Weber, T., and Kovacs, A.T. (2021). Phylogenetic Distribution of Secondary Metabolites in the Bacillus subtilis Species Complex. mSystems 6, e00057-00021. DOI: 10.1128/mSystems.00057-21.

46. Schmid, J.C., Frey, K., Scheiner, M., Garzon, J.F.G., Stafforst, L., Fricke, J.N., Schuppe, M., Schiewe, H., Zeeck, A., Weber, T., et al. (2021). The structure of cyclodecatriene collinolactone, its biosynthesis and semisynthetic analogues: Effects of monoastral phenotype and protection from intracellular oxidative stress. Angew. Chem. Int. Ed. Engl. 60, 23212-23216. DOI: 10.1002/anie.202106802.

47. Mingyar, E., Mühling, L., Kulik, A., Winkler, A., Wibberg, D., Kalinowski, J., Blin, K., Weber, T., Wohlleben, W., and Stegmann, E. (2021). A Regulator Based “Semi-Targeted” Approach to Activate Silent Biosynthetic Gene Clusters. Int. J. Mol. Sci. 22, 7567. DOI: 10.3390/ijms22147567.

48. Kontou, E.E., Gren, T., Ortiz-Lopez, F.J., Thomsen, E., Oves-Costales, D., Diaz, C., de la Cruz, M., Jiang, X., Jørgensen, T.S., Blin, K., et al. (2021). Discovery and Characterization of Epemicins A and B, New 30-Membered Macrolides from Kutzneria sp. CA-103260. ACS Chem. Biol. 16, 1456-1468. DOI: 10.1021/acschembio.1c00318.

49. Kiesewalter, H.T., Lozano-Andrade, C.N., Wibowo, M., Strube, M.L., Maroti, G., Snyder, D., Jørgensen, T.S., Larsen, T.O., Cooper, V.S., Weber, T., and Kovacs, A.T. (2021). Genomic and Chemical Diversity of Bacillus subtilis Secondary Metabolites against Plant Pathogenic Fungi. mSystems 6, e00770-00720. DOI: 10.1128/mSystems.00770-20.

50. Kautsar, S.A., Blin, K., Shaw, S., Weber, T., and Medema, M.H. (2021). BiG-FAM: the biosynthetic gene cluster families database. Nucleic Acids Res. 49, D490-D497. DOI: 10.1093/nar/gkaa812.

51. Jørgensen, T.S., Gren, T., Oves-Costales, D., Ortiz-Lopez, F.J., Carretero-Molina, D., Gonzalez, I., Blin, K., Genilloud, O., and Weber, T. (2021). Complete Genome Sequence of Amycolatopsis sp. CA-230715, Encoding a 35-Module Type I Polyketide Synthase. Microbiol Resour Announc 10, e0080521. DOI: 10.1128/MRA.00805-21.

52. Jørgensen, T.S., Gren, T., Kontou, E.E., Gonzalez, I., Roman-Hurtado, F., Oves-Costales, D., Thomsen, E., Charusanti, P., Genilloud, O., and Weber, T. (2021). Complete Genome Sequence of the Rare Actinobacterium Kutzneria sp. Strain CA-103260. Microbiol Resour Announc 10, e0049921. DOI: 10.1128/MRA.00499-21.

53. Jiang, X., Radko, Y., Gren, T., Palazzotto, E., Jørgensen, T.S., Cheng, T., Xian, M., Weber, T., and Lee, S.Y. (2021). Distribution of epsilon-Poly-L-lysine Synthetases in Coryneform Bacteria Isolated from Cheese and Human Skin. Appl. Environ. Microbiol. 87, e01841-01820. DOI: 10.1128/AEM.01841-20.

54. Gren, T., Whitford, C.M., Mohite, O.S., Jørgensen, T.S., Kontou, E.E., Nielsen, J.B., Lee, S.Y., and Weber, T. (2021). Characterization and engineering of Streptomyces griseofuscus DSM 40191 as a potential host for heterologous expression of biosynthetic gene clusters. Scientific Reports 11, 18301. DOI: 10.1038/s41598-021-97571-2.

55. Dolya, B., Busche, T., Jørgensen, T.S., Ochi, K., Gren, T., Weber, T., Kalinowski, J., Luzhetskyy, A., and Ostash, B. (2021). A database of sequenced genomes of different Streptomyces albus J1074 strains and uses thereof. Visnyk of Lviv University. Biological series, 26-34. DOI: 10.30970/vlubs.2021.85.03.

56. Carretero-Molina, D., Ortiz-Lopez, F.J., Gren, T., Oves-Costales, D., Martin, J., Roman-Hurtado, F., Jorgensen, T.S., de la Cruz, M., Diaz, C., Vicente, F., et al. (2021). Identification of the 216kbp gene cluster and structure elucidation of gargantulides B and C, new complex 52-membered macrolides from Amycolatopsis sp. BioRxiv, https://doi.org/10.1101/2021.1108.1111.455926. DOI: 10.1101/2021.08.11.455926.

57. Blin, K., Shaw, S., Kloosterman, A.M., Charlop-Powers, Z., van Wezel, G.P., Medema, M.H., and Weber, T. (2021). antiSMASH 6.0: improving cluster detection and comparison capabilities. Nucleic Acids Res. 49, W29-W35. DOI: 10.1093/nar/gkab335.

58. Blin, K., Shaw, S., Kautsar, S.A., Medema, M.H., and Weber, T. (2021). The antiSMASH database version 3: increased taxonomic coverage and new query features for modular enzymes. Nucleic Acids Res. 49, D639-D643. DOI: 10.1093/nar/gkaa978.

59. Beck, C., Gren, T., Ortiz-López, F.J., Jørgensen, T.S., Carretero-Molina, D., Martín Serrano, J., Tormo, J.R., Oves-Costales, D., Kontou, E.E., Mohite, O.S., et al. (2021). Activation and Identification of a Griseusin Cluster in Streptomyces sp. CA-256286 by Employing Transcriptional Regulators and Multi-Omics Methods. Molecules 26, 6580. DOI: 10.3390/molecules26216580.

60. Beck, C., Gren, T., Jørgensen, T.S., Gonzáles, I., Román-Hurtado, F., Oves-Costales, D., Genilloud, O., and Weber, T. (2021). Complete Genome Sequence of Strain CA-256286. Microb Resour Announc 10, e0029021. DOI: 10.1128/MRA.00290-21.

61. Beck, C., Blin, K., Gren, T., Jiang, X., Mohite, O.S., Palazzotto, E., Tong, Y., Charusanti, P., and Weber, T. (2021). Metabolic Engineering of Filamentous Actinomycetes. In Metabolic Engineering: Concepts and Applications, J. Nielsen, G. Stephanopoulos, and S.Y. Lee, eds. (Wiley-VCH GmbH), pp. 653-688. 10.1002/9783527823468.ch17.

62. Yang, D., Park, S.Y., Park, Y.S., Eun, H., and Lee, S.Y. (2020). Metabolic Engineering of Escherichia coli for Natural Product Biosynthesis. Trends Biotechnol. 38, 745-765. DOI: 10.1016/j.tibtech.2019.11.007.

63. Tong, Y., Whitford, C.M., Blin, K., Jørgensen, T.S., Weber, T., and Lee, S.Y. (2020). CRISPR-Cas9, CRISPRi and CRISPR-BEST-mediated genetic manipulation in streptomycetes. Nat. Protoc. 15, 2470-2502. DOI: 10.1038/s41596-020-0339-z.

64. Tong, Y., Jørgensen, T.S., Whitford, C.M., Weber, T., and Lee, S.Y. (2020). CRISPR-nRAGE, a Cas9 nickase-reverse transcriptase assisted versatile genetic engineering toolkit for E. coli. BioRxiv, https://doi.org/10.1101/2020.1109.1102.279141. DOI: 10.1101/2020.09.02.279141.

65. Tong, Y., and Deng, Z. (2020). An aurora of natural products-based drug discovery is coming. Synth. Syst. Biotechnol. 5, 92-96. DOI: 10.1016/j.synbio.2020.05.003.

66. Steinke, K., Mohite, O.S., Weber, T., and Kovács, Á.T. (2020). Phylogenetic distribution of secondary metabolites in the Bacillus subtilis species complex. BioRxiv, https://doi.org/10.1101/2020.1110.1128.358507. DOI: 10.1101/2020.10.28.358507.

67. Mungan, M.D., Alanjary, M., Blin, K., Weber, T., Medema, M.H., and Ziemert, N. (2020). ARTS 2.0: feature updates and expansion of the Antibiotic Resistant Target Seeker for comparative genome mining. Nucleic Acids Res. 48, W546-W552. DOI: 10.1093/nar/gkaa374.

68. Kautsar, S.A., Blin, K., Shaw, S., Navarro-Munoz, J.C., Terlouw, B.R., van der Hooft, J.J.J., van Santen, J.A., Tracanna, V., Suarez Duran, H.G., Pascal Andreu, V., et al. (2020). MIBiG 2.0: a repository for biosynthetic gene clusters of known function. Nucleic Acids Res. 48, D454-D458. DOI: 10.1093/nar/gkz882.

69. Jiang, X., Palazzotto, E., Wybraniec, E., Munro, L.J., Zhang, H., Kell, D.B., Weber, T., and Lee, S.Y. (2020). Automating Cloning by Natural Transformation. ACS Synth Biol 9, 3228-3235. DOI: 10.1021/acssynbio.0c00240.

70. Gren, T., Whitford, C.M., Mohite, O.S., Jørgensen, T.S., Kontou, E.E., Nielsen, J.B., Lee, S.Y., and Weber, T. (2020). Characterization and Engineering of Streptomyces griseofuscus DSM 40191 as a Potential Host for Heterologous Expression of Biosynthetic Gene Clusters. BioRxiv, https://doi.org/10.1101/2020.1111.1106.372458. DOI: 10.1101/2020.11.06.372458.

71. Gren, T., Jørgensen, T.S., Whitford, C.M., and Weber, T. (2020). High-Quality Sequencing, Assembly, and Annotation of the Streptomyces griseofuscus DSM 40191 Genome. Microbiology Resource Announcements 9, 9:e01100-01120. DOI: 10.1128/mra.01100-20.

72. Choi, K.R., and Lee, S.Y. (2020). Protocols for RecET-based markerless gene knockout and integration to express heterologous biosynthetic gene clusters in Pseudomonas putida. Microb Biotechnol 13, 199-209. DOI: 10.1111/1751-7915.13374.

73. Blin, K., Shaw, S., Tong, Y., and Weber, T. (2020). Designing sgRNAs for CRISPR-BEST base editing applications with CRISPy-web 2.0. Synth Syst Biotechnol 5, 99-102. DOI: 10.1016/j.synbio.2020.05.005.

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