An important aim of the iimena project is to develop methods that enable us to more efficiently get access to the potential new antibiotics produced by actinomycetes. One of the most important developments in the recent years have been molecular tools using CRISPR technologies. iimena scientists have been on the forefront of developing such tools and sharing them with the international research community. We are therefore very happy that a protocol now has been published in Nature Protocols. In the publication, titled "CRISPR–Cas9, CRISPRi and CRISPR-BEST-mediated genetic manipulation in streptomycetes", users of the toolkit find very detailed step-by-step guides on how to most efficiently use our toolkit. Link to the protocol:Tong, Y. et al. CRISPR-Cas9, CRISPRi and CRISPR-BEST-mediated genetic manipulation in streptomycetes. Nat. Protoc., doi:10.1038/s41596-020-0339-z (2020). Link to interactive PDF…
We are looking forward to host the 3rd International Workshop for early career scientists on Genome Mining for Natural Products, which will take place from 12.-14. September at DTU Biosustain. 30 participants from 8 different countries will get two days of intensive training using the state of the art bioinformatics tools antiSMASH and NORINE and will have the opportunity to directly interact with the developers of the software.
Scientists working in the iimena-project just published a review article on the potential of genome-scale metabolic reconstructions for optimizing antibiotics biosynthesis in actinomycetes. The article, which was published in the "Biotechnolgy J." can be downloaded here or requested from the authors.
The positions are now filled. More about DTU Biosustain: http://biosustain.dtu.dk Position 1 Filled: "Identification and characterization of antibiotics biosynthetic pathways" Click here to find the full description: Posting at DTU job portal Position 2 Filled : “In silico modelling of metabolism to understand and facilitate natural products biosynthesis” Click here to find the full description: Posting at DTU job portal Position 3 Filled: “Omics-based analysis of the regulation of secondary metabolism in actinomycetes” Click here to find the full description: Posting at DTU job portal Application deadline for all three positions is Aug 20th, 2017 Please consult the DTU PhD guide for admission criteria as a PhD student at DTU.