Scientists from DTU Biosustain and KAIST funded by Novo Nordisk Foundation and part of the iimena project have developed a new CRISPR-prime editing toolkit to engineer E. coli.
Have a look at the Nature Communications paper -> A versatile genetic engineering toolkit for E. coli based on CRISPR-prime editing. nature Communications. 2021. DOI:10.1038/s41467-021-25541-3
