NEW AND HIGHLY COMPLEX ANTIBACTERIAL MACROLIDES FROM AMYCOLATOPSIS SP.

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As a result of the IIMENA project, we now report the discovery of gargantulides B and C, which are among the largest and most complex bacterial polyketides discovered so far. These huge macrolactones were isolated from an Amycolatopsis sp. strain during an antibacterial screening campaign and their structures were fully elucidated based on 2D NMR spectroscopy. Subsequent identification and analysis of their extraordinarily large biosynthetic gene cluster, together with extensive NMR-based analyses, allowed us to completely assign and revise the absolute stereochemistry of the entire gargantulide family. The new macrolides showed potent antibacterial activity against Gram-positive bacteria including methicillin-resistant Staphylococcus aureus (MRSA) and vancomycin-resistant Enterococcus (VRE), and moderate but unusual activity against the clinically relevant Gram-negative pathogen Acinetobacter baumannii. If you want to know more about these interesting bioactive compounds…
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New CRISPR-prime editing toolkit to engineer E. coli

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Scientists from DTU Biosustain and KAIST funded by Novo Nordisk Foundation and part of the iimena project have developed a new CRISPR-prime editing toolkit to engineer E. coli. Have a look at the Nature Communications paper -> A versatile genetic engineering toolkit for E. coli based on CRISPR-prime editing. nature Communications. 2021. DOI:10.1038/s41467-021-25541-3
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NEW METABOLITES PRODUCED FROM A RARE ACTINOMYCETE

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As part of the iimena project, we are investigating the genomes, as well as the metabolite profiles of many actinomycete strains. Despite the well-known Streptomyces, there are other underexplored genera in the phylum of Actinobacteria that are very promising on producing new, complex specialized metabolites. Through a combined genetic and metabolite approach, we investigated a rare Kutzneria strain that proved to produce two novel macrolides, epemicins A and B. The work, that is now published in ACS Chemical Biology, was a collaboration between the chemistry team of the iimena project, that isolated and structurally elucidated the new compounds, as well as the genome mining and engineering team, that managed to link epemicins to the gene cluster responsible for their biosynthesis. Link to the paper: Discovery and Characterization of Epemicins A…
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IIMENA Project Half-Annual Meeting

IIMENA Project Half-Annual Meeting

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Today we had the iimena annual scientific project meeting, again online due to the COVID-19 situation, with all the partners from the IIMENA project funded by a Challenge Grant of the Novo Nordisk Foundation. The meeting allowed the partners to present their latest update on the results from the project as well as the next steps and plans for the future. We hope the next meeting towards the end of the year 2021 will be face-to-face.
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CPH Bioscience Lecture: The AMR Challenge – Mining soils for drugs – Microbes as source for novel antibiotics

CPH Bioscience Lecture: The AMR Challenge – Mining soils for drugs – Microbes as source for novel antibiotics

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On 25th February 2021 at the Novo Nordisk Foundation, professor Tilmann Weber had a pleasure to give a fantastic talk with title Mining soils for drugs – Microbes as source for novel antibiotics as part of the CPH Bioscience lecture. He presented the newest research result from his group at CFB as well as from the IIMENA project where he has a role of coordinator.
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